对照。[结论]经除草剂筛选和PCR对T<sub>0</sub>和T<sub>1</sub>代幼苗检测，初步证明获得了转化植株。
关键词 植物转化;燕麦;玉米;高粱
中图分类号 S188 文献标识码 A 文章编号 0517-6611（2015）03-025-04
Trauma Embryo of Genetic Transformation System with Agrobacterium Mediated Method
ZHANG Lijun1，2， LIU Longlong<sup>1</sup>，ZHANG Jianzhen<sup>2</sup>， CUI Lin1* et al
（1. Crop germplasm resources research institute， Shanxi Academy of Agricultural Sciences; Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau， Ministry of Agriculture， P. R. China， Taiyuan， Shanxi 030031;2. Research Institute of Applied Biology，Shanxi University，Taiyuan，Shanxi 030001）
Key words [Objective]The purpose was to optimize agrobacteriummediated plant germination seed transformation method，increase conversion rate， breed GM varieties with specific functions， and improve viability and adaptability of crops. [Method]This paper consulted agrobacteriummediated plant germination seed transformation method and selected germinating seeds of oat， maize and sorghum as test materials. Germinating seeds of oat， maize and sorghum were infected and transformed by agrobacterium strain， which included plasmid P3301ubiAc. Transformation conditions were optimized. Genetic transformation systems of oat， maize and sorghum were studied. Concentration of bacterium， seed germinating time， ultrasonic power， and influence of concentration of acetosyringone and glufosinate on the above three crops were compared and analyzed. [Result]The experiment showed that when 0.6>OD600≥0.4， transformation achieved the best effect; Ultrasonic power was 900 W at the first time and treatment time was 10 minutes; Ultrasonic power was 200 W at the second time and transformation achieved the best effect when treatment time was 4 minutes; Acetosyringone could affect germination of oat， sorghum and maize significantly. It could increase transformation rate of maize and lower that of sorghum， but for oat， its effect was not obvious; in terms of tolerance to glufosinate， the difference was not significant among transformed plants of oat， maize and sorghum. Compared with nontransformed plants， seed germination of oat， maize and sorghum were restrained to some degree under the stress of 0.8 mg/L herbicide solution. With 1 mg/L herbicide solution to select seedlings of T<sub>0</sub> and T<sub>1</sub> transformed plants， the result showed survival rates of transformed plants is higher than those of control group. [Conclusion ]After herbicide selection and PCR test on T<sub>0</sub> and T<sub>1</sub> seedlings， it was proved preliminarily that transformed plants were got.